protag-HiRes anti-GFP-X4 AF568

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Cat. No: 81411L

Available, delivery time 1-7 days

$504.00*

Key Features
  • AF568 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with acGFP, Cerulean, Citrine, eCFP, GFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate AF568
Application ICC/IF
Purification Affinity chromatography
Reactivity Cerulean, Citrine, EGFP, Emerald GFP, GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1H1/1B2
Immunogen GFP
Formulation 1.25 µM of each fluorescently labeled single-domain antibody clone in buffered saline, 50% glycerol, 0.09% sodium azide. 5 µM total fluorophore concentration
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500
Background

protag-HiRes anti-GFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, acGFP, GFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.

In protag-HiRes anti-GFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-GFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our protag-HiRes anti-GFP-X4 products excellent tools for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.