protag-HiRes anti-GFP-X4 Atto 488
- Atto 488 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with acGFP, Cerulean, Citrine, eCFP, GFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | Atto 488 |
| Application | ICC/IF |
| Purification | Affinity chromatography |
| Reactivity | Cerulean, Citrine, EGFP, Emerald GFP, GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire |
| No reactivity | Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 1H1/1B2 |
| Immunogen | GFP |
| Formulation | 1.25 µM of each fluorescently labeled single-domain antibody clone in buffered saline, 50% glycerol, 0.09% sodium azide. 5 µM total fluorophore concentration |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
Background
protag-HiRes anti-GFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, acGFP, GFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.
In protag-HiRes anti-GFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-GFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our protag-HiRes anti-GFP-X4 products excellent tools for all microscopy techniques.
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FAQs
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.