protag-HiRes anti-GFP-X4 Atto 542

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Cat. No: 81406L

Available, delivery time 1-7 days


Key Features
  • Atto 542 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with acGFP, Cerulean, Citrine, eCFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Atto 542
Application ICC/IF
Purification Affinity chromatography
Reactivity Cerulean, Citrine, EGFP, Emerald GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1H1/1B2
Immunogen GFP
Formulation 1.25 µM of each fluorescently labeled single-domain antibody clone in buffered saline, 50% glycerol, 0.09% sodium azide. 5 µM total fluorophore concentration
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:250

protag-HiRes anti-GFP is a blend of two camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.

In protag-HiRes anti-GFP-X4, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. The blend of two camelid single-domain antibodies therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our protag single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our protag-HiRes anti-GFP-X4 can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our protag-HiRes anti-GFP-X4 products excellent tools for all microscopy techniques.

Category Protocol
Size 805.06 KB
Filetype pdf
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