protag-HiRes anti-TagFP-X1 Atto 488
- Atto 488 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with mKate, mKate2, mTagBFP, mTagRFP, mTagRFP657
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | Atto 488 |
| Application | ICC/IF |
| Purification | Affinity chromatography |
| Reactivity | mKate/mKate2, mTagBFP, mTagRFP, mTagRFP657 |
| No reactivity | Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, dsRed or their most common derivatives |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 1H7 |
| Immunogen | TagFP |
| Formulation | 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
Background
protag-HiRes anti-TagFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes fluorescent proteins derived from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657, mKate and mKate2 with high affinity and specificity.
In protag-HiRes anti-TagFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Downloads
Q & A's
Customer Reviews
Login
FAQs
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.