protag-HiRes anti-TagFP-X2 AF568
- AF568 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with mKate, mKate2, mTagBFP, mTagRFP, mTagRFP657
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | AF568 |
| Application | ICC/IF |
| Purification | Affinity chromatography |
| Reactivity | mKate/mKate2, mTagBFP, mTagRFP, mTagRFP657 |
| No reactivity | Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, dsRed or their most common derivatives |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 1H7 |
| Immunogen | TagFP |
| Formulation | 2.5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
Background
protag-HiRes anti-TagFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes fluorescent proteins derived from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657, mKate and mKate2 with high affinity and specificity.
In protag-HiRes anti-TagFP-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-TagFP-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-TagFP-X2 can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - render the protag-HiRes anti-TagFP-X2 products superior tools for all microscopy techniques.
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FAQs
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.