protag-HiRes anti-TagFP-X2 Sulfo-Cyanin 5 (Cy5)

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Cat. No: 83210L

Available, delivery time 1-7 days


Key Features
  • Sulfo-Cyanin 5 (Cy5) conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with mKate, mKate2, mTagBFP, mTagRFP, mTagRFP657

Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Sulfo-Cyanin 5 (Cy5)
Application ICC/IF
Purification Affinity chromatography
Reactivity mKate/mKate2, mTagBFP, mTagRFP, mTagRFP657
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, dsRed or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1H7
Immunogen TagFP
Formulation 2.5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500

protag-HiRes anti-TagFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes fluorescent proteins derived from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657, mKate and mKate2 with high affinity and specificity.

In protag-HiRes anti-TagFP-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-TagFP-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-TagFP-X2 can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - render the protag-HiRes anti-TagFP-X2 products superior tools for all microscopy techniques.

Category Protocol
Size 805.06 KB
Filetype pdf
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