protag-HiRes anti-mScarlet-i-X2 Sulfo-Cyanin 3 (Cy3)

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Cat. No: 84209L

Available, delivery time 1-7 days

$504.00*

Key Features
  • Sulfo-Cyanin 3 (Cy3) conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with dsRed1, dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Sulfo-Cyanin 3 (Cy3)
Application ICC/IF
Purification Affinity chromatography
Reactivity dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 2B12
Immunogen RFP
Formulation 2.5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500
Background

protag-HiRes anti-mScarlet-i camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes mScarlet-i in its native conformation with high affinity and specificity. It can also recognize some mRFP-derived red fluorescent protein like mOrange2, dsRed, tdTomato, mRFP and mCherry in its native conformation. It does not cross-react with GFP or mTagBFP derivatives.

In protag-HiRes anti-mScarlet-i-X2, two fluorophore molecules are site-specifically coupled to each individual single-domain antibody. protag-HiRes anti-mScarlet-i-X2 can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our single-domain antibodies, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, the protag-HiRes anti-mScarlet-i-X2 can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - render the protag-HiRes anti-mScarlet-i-X2 products superior tools for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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