protag-HiSec anti-chicken IgY-X2 Atto 488
Indirect immunostaining of PFA fixed mouse brain section with chicken anti-MAP2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (green). Nuclei have been visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Mouse brain section with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Mouse cerebellum section stained with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Indirect immunostaining of PFA fixed mouse brain section with chicken anti-MAP2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (green). Nuclei have been visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Mouse brain section with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Mouse cerebellum section stained with chicken anti-MAP 2 and protag-HiSec anti-chicken IgY-X2 Sulfo-Cyanin 5 (Cy5)(green). Nuclei were stained using DAPI (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Key Features
- Atto 488 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF and IHC
- Reacts with chicken IgY
Product description
| Quantity | 500 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | Atto 488 |
| Application | ICC/IF, IHC |
| Purification | Affinity chromatography |
| Reactivity | Chicken IgY |
| No reactivity | Goat IgG, Guinea pig IgG, Mouse IgG, Rabbit IgG, Rat IgG |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 3G2 |
| Immunogen | Chicken IgY |
| Formulation | 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
| Immunohistochemistry (IHC) - frozen | 1:500 |
| Immunohistochemistry (IHC) - paraffin | 1:500 |
Background
protag-HiSec anti-chicken IgY is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against chicken IgY and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary chicken antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.