protag-HiSec anti-mouse IgG kLC-X2 Licor 680RD

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Cat. No: 80213

Available, delivery time 1-7 days

$216.00*

Key Features
  • Licor 680RD conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF, IHC, FACS and WB
  • Reacts with mouse IgG kappa

Product description
Quantity 500 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Licor 680RD
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Mouse IgG kappa light chain
No reactivity Chicken IgG, Goat IgG, Guinea pig IgG, Rabbit IgG, Rat IgG
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1A23
Immunogen Mouse IgG kLC
Formulation 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500
Immunohistochemistry (IHC) - frozen 1:500
Immunohistochemistry (IHC) - paraffin 1:500
Flow Cytometry (FACS) 1:500
Western Blot (WB) 1:500
Background
protag-HiSec anti-mouse IgG is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against mouse IgG and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.
Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.