protag-HiSec anti-mouse IgG1-X2 Alexa 647
Multiplexing: Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-MAP2 primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 488 (Cat. No. 80605, represented in red), an anti-GFAP primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 647N (represented in green) and an anti-Synapsin1 primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 565 (represented in yellow). Nuclei were visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Multiplexing: Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-MAP2 primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 488 (Cat. No. 80605, represented in red), an anti-GFAP primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 647N (represented in green) and an anti-Synapsin1 primary antibody detected with protag-HiSec anti-mouse IgG1-X2 Atto 565 (represented in yellow). Nuclei were visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Key Features
- Alexa 647 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF and WB
- Reacts with mouse IgG1
Product description
| Quantity | 500 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | Alexa 647 |
| Application | ICC/IF, WB |
| Purification | Affinity chromatography |
| Reactivity | Mouse IgG1 |
| No reactivity | Chicken IgG, Goat IgG, Guinea pig IgG, Rabbit IgG, Rat IgG |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 10A4 |
| Immunogen | Mouse IgG1 |
| Formulation | 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide (10 µM fluorophore) |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
| Western Blot (WB) | 1:500 |
Background
protag-HiSec anti-mouse IgG1 is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against mouse IgG1 and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.
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