protag-HiSec anti-mouse IgG2a/b-X2 Atto 488

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Cat. No: 80505

Available, delivery time 1-7 days

$216.00*

Key Features
  • Atto 488 conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF and WB
  • Reacts with mouse IgG2a and IgG2b

Product description
Quantity 500 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Atto 488
Application ICC/IF, WB
Purification Affinity chromatography
Reactivity Mouse IgG2 and IgG2b
No reactivity Chicken IgG, Donkey IgG, Goat IgG, Guinea pig IgG, Human IgG, Mouse IgG1 and IgM, Rabbit IgG, Rat IgG
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 14A4
Immunogen Mouse IgG2
Formulation 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide (10 µM fluorophore)
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500
Western Blot (WB) 1:500
Background
protag-HiSec anti-mouse IgG2a/b is an isotype- and species-specific camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It is directed against mouse IgG2a and IgG2b and contains two site-specifically coupled fluorophores per protag molecule. One single-domain antibody targets two fluorophores to a primary mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. There are no batch-to-batch variations, since the protag-HiSecs single-domain antibodies are recombinantly expressed.
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