Disease related

anti-Histone H1 mouse monoclonal, AE-4, purified

Cat. No: 691531

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human, mouse and rat
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
Storage 2-8°C
Intended use Research use only
Clone AE-4
Immunogen Human leukemia biopsy cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P07305 (Human), P10922 (Mouse), P43278 (Rat)
Synonym Histone H1.0, Histone H1', Histone H1(0 [Cleaved into: Histone H1.0, N-terminally processed], H1-0, H1F0, H1FV
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Background

AE-4 recognizes histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells.

Positive control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma or tonsil.

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.