AAV Capsid Protein Antibodies

Detect viral capsid proteins VP1, VP2 and VP3

PROGEN's AAV capsid protein antibodies recognize linear epitopes of the viral capsid proteins VP1, VP2 and VP3. The high sequence similiarity of AAV capsid proteins allows the easy detection of proteins across serotypes.

Figure 1 shows the three capsid proteins and the corresponding antibody binding sites. As all VP proteins are encoded by the same reading frame they only differ in their N-terminal region due to the use of an alternate start codon and alternate splicing. Antibodies binding the C-terminal part, e.g. B1, are able to bind to all of the capsid proteins whereas antibodies like A1 can only bind to VP1 and A69 to VP1 and VP2.

Capsid protein antibodies can be used in western blot analyses to detect the expression levels of VP1, VP2 and VP3, as well as for protein localization studies or protein-protein interactions using chromatography, immunoprecipitation or immunofluorescence.

View AAV Capsid Protein Antibodies

Binding Sites and Reactivity

Capsid protein antibodies were raised in mice against AAV2 capsid proteins and are suitable for all applications detecting linear epitopes of the denatured VP protein, such as western blot analysis.

Serotype	Antibody	Residues*	Binding Site
AAV2	A1	123-KRVLEPLGL-131	Binding site is located at the N-terminus of the VP1 protein. Detection of VP1 only.
AAV2	A69	171-LNFGQTGDADSV-182	Binding site is located at the N-terminus of the VP2 protein. Detection of VP1 and VP2.
AAV2	B1	726-IGTRYLTR-733	Binding site is located at the C-terminus common to all three VP proteins. Detection of VP1, VP2 and VP3.
*The amino acid position is with regards to the VP1 sequence.
Epitope mapping experiments (Wobus et al., 2000) identified aa123 to aa131 (N-terminus;) as the specific binding region for the monoclonal A1 antibody. Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

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Cross-reactivity

Antibody

AAV1

AAV2

AAV3

AAV4

AAV5

AAV6

AAV7

AAV8

AAV9

AAVrh10

AAV11

AAV12

AAVDJ

A1

x

A69

x

na

x

B1

x

VP51

na

x

na

x

na

x - Reactivity
na - Not analyzed
Empty field - no reactivity

A1

Reacts with VP1 proteins from all common serotypes AAV1-12. In immunoprecipitation a reaction with a non-AAV derived protein can occasionally be seen.

View AAV Capsid Protein Antibodies

B1

The B1 antibody shows high cross-reactivity with most of the tested AAV serotypes due to high sequence similarities of VP proteins derived from different AAVs.

Using AFDyes

Using an AFDye™ labeled antibody allows you to detect your proteins of interest without using a secondary antibody. This saves you valuable time as you can also simultaneously detect different proteins in the same sample by using differently labelled antibodies e.g. AFDye™ 647 and AFDye™ 488.

PROGEN offers two different AFDye™ conjugates for the B1 antibody: AFDye™ 647 and AFDye™ 488.

AFDye^TM 647

Appearance	Bright deep red fluorescent
Excitation	648 nm
Emission	671 nm
Filterset	E.g. Cy5
Spectrally similar Dyes	Alexa Fluor® 647
Advantage	Low autofluorescence due to long wavelength