Antibodies for the detection of AAV

PROGEN antibodies are useful for the characterization of different stages of an AAV infection and effective in the analyses of the AAV assembly process. There are two main categories of AAV antibodies:

  1. AAV capsid protein antibodies that specifically react with denatured AAV capsid proteins and thereby recognize the linear epitopes of the viral capsids.
  2. AAV particle antibodies that specifically react with intact AAV particles, i.e. empty and full capsids and thereby recognize a conformational epitope of the assembled capsids of certain serotypes. 

PROGEN also offers a selection of AAV replicase antibodies.


View AAV Antibodies

Using AAV Antibodies for AAV-based Gene Therapy

This short video gives an overview of capsid and particle antibodies and how they can be used in your next AAV gene therapy project. 

AAV Capsid Protein Antibodies

The AAV capsid protein antibodies recognize linear epitopes of the viral capsid proteins that consists of neighboring amino acids. These epitopes have high sequence similarity and therefore the antibodies detect the viral capsid proteins VP1, VP2 and VP3 across the serotypes.

On the left hand side you can see the primary sequences of the three capsid proteins and the binding sites along the sequence. Since all proteins are encoded by the same gen they only differ in alternate splicing. Therefore antibody B1 is able to bind all capsid proteins whereas the binding site of antibody A1 is only located on VP1. For the same reason antibody A69 is only suitable for VP1 and VP2.

All of the antibodies are useful to study the expression levels of VP1, VP2 and VP3 in western blot analyses, in order to determine the protein localizations or protein-protein interactions by affinity chromatography, immunoprecipation or immunofluorescence.

For more information on our specific AAV capsid protein antibodies, please see our VP1, VP1/VP2 and VP1/VP2/VP3 categories below.
Some of the following antibodies are also available in sample sizes of 10µg (lyophilized).

AAV Particle Antibodies

The AAV particle antibodies recognize conformational epitopes of the viral capsid proteins that are only present on fully assembled capsids. These epitopes consist of distant amino acids either of the same protein or of different proteins that are in close proximity, and they might vary across the different serotypes. However, for some of the antibodies some cross-reactivity is known (for more information, please see the specific AAV particle antibodies by AAV serotype below). The AAV particle antibodies are useful for the following processes:

  • to study fully assembled and intact capsids by dot blot analyses
  • to quantify the intact viral capsids (empty and full) by ELISA
  • to detect neutralizing antibodies in patients serum
  • to determine protein-protein interaction or to purify virus preparations by affinity chromatography or immunoprecipitations
  • to analyze virus particle localizations by immunofluorescence.
  • Some of the following antibodies are also available in sample sizes of 10µg (lyophilized). 

AAV Replicase Antibodies

PROGEN also offers antibodies reacting with the four regulatory proteins Rep78, Rep68, Rep52 and Rep40. These proteins are encoded by the rep-gene, playing an important role in AAV genome replication and packing. During AAV Infection mentioned proteins are always expressed in different concentrations in human cells. Check out the table below which antibody suits your demand.


Antibody

Rep78

Rep68

Rep52

Rep40

Cat. No.

anti-AAV2 Replicase mouse monoclonal, 76.3, lyophilized, purified X X 61073
anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant X* X* X* X* 65172
anti-AAV2 Replicase mouse monoclonal, 259.5, lyophilized, purified X X X X 61071
anti-AAV2 Replicase mouse monoclonal, 303.9, lyophylized, purified X X X X 61069

X: reacts with
X*: shows low affinity

AAV Antibodies

Yes, PROGEN offers several AAV virus particle specific, neutralizing antibodies against AAV serotypes 1, 2, 4, 5, 6, 8, and 9.
For more information on the neutralizing activity of our antibodies check out the internally collected data and publications for the corresponding serotypes. Just click the button for your specific serotype after following the link.
Yes, PROGEN offers a selection of AAV antibodies in sample sizes. Find a list of antibodies available in sample size of 10 µg (lyophilized) here:  AAV Antibodies
PROGEN offers a wide range of AAV antibodies in different quantities and formats (e.g. lyophilized and supernatant). A selection of AAV antibodies are also available in 10µg lyophilized sample sizes. For more information on the different AAV antibodies please see AAV Antibodies.

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes



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