The progress into clinical trials as well as the increasing demand for AAV gene therapy vectors in the field in general leads to an increasing need of high yields, high purity and high quality AAV particles. Therefore, it gets more and more important to ensure a comprehensive monitoring during the vector manufacturing process. Monitoring the VP1, VP2 and VP3 expression levels as well as the expression ratios of the AAV capsid proteins allow to optimize the yield and the potency of the production at an early time point.
The AAV2 VP protein standard can be used to create mixtures of VP1, VP2 and VP3 with a precise molar ratio, e.g. 1:1:10 for comparison with the protein composition of your sample by protein detection methods like western blot. All three AAV2 proteins are available as individual proteins (10 µg each) and can be used to prepare specific VP protein mixtures as needed.
AAV2 VP Protein Standards (100 µg/ml)