AAV Particle Antibodies
Recognize fully assembled
capsids easily
AAV particle antibodies recognize conformational epitopes on viral capsids that are only present on fully assembled capsids. These epitopes are made up of distant amino acids either from the same protein or from different proteins that are close by.
You can use particle antibodies to
Analyze: track the location of virus particles and identify protein-protein interactions
Purify: suitable for AAV particle purification using affinity chromatography
Characterize: determine your AAV capsid titers
Control: analyze the condition of your AAV particles
Monitor: use as a positive control for neutralization assays with patient sera
Cross Reactivity & Neutralization
All our AAV particle antibodies were tested for their cross-reactivity and some showed high cross-reactivity with other serotypes. Use the table below to see the cross reactivity.
Click on the buttons in the first column to get more product information including specific binding sites, neutralizing activity and available products.
Antibody Clone |
AAV1 |
AAV2 |
AAV3 |
AAV4 |
AAV5 |
AAV6 |
AAV7 |
AAV8 |
AAV9 |
AAVrh10 |
AAV11 |
AAAV12 |
AAVrh74 |
Anc80 |
AAVDJ |
AAV2.7m8 |
ADK1a |
✓ | ✓ | ✓ | na | na | na | ||||||||||
A20 |
✓ | ✓ | na | na | ✓ | |||||||||||
A20R |
✓ | ✓ | ✓ | ✓ | ||||||||||||
ADK4 |
✓ | na | na | na | ||||||||||||
ADK5a |
✓ | na | na | na | ||||||||||||
ADK5b |
✓ | na | na | na | na | na | na | |||||||||
ADK6 |
✓ | na | na | na | ||||||||||||
ADK8 |
✓ | ✓ | ✓ | (✓) | ✓ | ✓ | na | |||||||||
ADK8/9 |
na | ✓ | (✓) | ✓ | na | na | ✓ | ✓ | (✓) | na | ||||||
| ADK9 | ✓ | na | na | na | na | |||||||||||
ADK9-1R |
na | ✓ | na | na | na | na | na | na |
✓ - Reactivity
(✓) - Weak Reactivity
na - Not analysed
Empty field - no reactivity
Data for assembled capsids AAV7/11/12 Under Courtesy of Regine Heilbronn, Charité Universitätsmedizin Berlin
Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212 222
What customers are saying
'All AAV primary antibodies I have ordered from PROGEN have been specific and reliable.'
Scientist I, ElevateBio
AAV Antibodies FAQs
Yes, our AAV particle antibodies detect conformational epitopes only present on assembled capsids.
You can find all AAV particle antibodies here.
In contrast, AAV capsid protein antibodies recognize linear epitopes present on denatured VP proteins.
You can find all AAV capsid protein antibodies here.
Yes, PROGEN offers a selection of AAV antibodies in 10 µg sample sizes. You can find all available AAV antibodies here: AAV Antibodies
Anti-Adeno-Associated Virus Capsid Proteins Antibodies
Anti-Adeno-Associated Virus Particle Antibodies
For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:
-
AAV 1
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794–1808 (2015).
- McCraw et al. Structureof adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology (2012) 431:40-9.
- Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
- Huttner et al. Genetic modifications of the adeno-associated virus type 2 capsid reduce the affinity and the neutralizing effects of human serum antibodies. Gene Ther (2003) 10:2139-47.
- Lochrie et al. Mutations on the external surfaces of adeno-associated virus type 2 capsids that affect transduction and neutralization. J Virol (2006) 80:821-34.
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794–1808 (2015).
- Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739–7751 (2012).
EM structures of assembled AAV1, 2, 4, 5, 6, and 8 capsids with Fab-fragments are available in the following publications:
- Emmanuel, S. N. et al. Parvovirus Capsid-Antibody Complex Structures Reveal Conservation of Antigenic Epitopes Across the Family. Viral Immunolgoy. 34(1) (2021).
- McCraw, S. M. et al. Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology. 432(1-2):40-9 (2012).
- Tseng, Y. et al. Adeno-Associated Virus Serotype 1 (AAV1)- and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J Virol. 89(3):1794-1808 (2015).
- Jose, A. et al. High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors. J Virol. 93(1):e01394-18 (2019).
- Silveria, M. A. et al. The Structure of an AAV5-AAVR Complex at 2.5 Å Resolution: Implications for Cellular Entry and Immune Neutralization of AAV Gene Therapy Vectors. Viruses. 12(11):1326 (2020).
AlphaLISA® AAV2 Capsid Detection Kit
PerkinElmer has integrated PROGEN’s well-established AAV2 particle antibody A20R into their no-wash AlphaLISA® assay technology. The AlphaLISA® AAV2 Capsid Detection Kit is designed to quantify AAV2 capsids in buffer, cell culture media, cell lysates and cell supernatants.
PROGEN’s A20R antibody exclusively detects fully assembled, intact AAV2 capsids, providing an efficient and reliable quantification of the overall AAV2 capsid titer.
AAV Capsid Protein Antibodies
React with linear epitopes of the denatured AAV capsid proteins VP1, VP2 and VP3
AAV Particle Antibodies
Recognize conformational epitopes of the viral capsid proteins that are only present on fully assembled capsids.
Human Chimeric AAV Antibodies
Reliable assay controls to be used when detecting AAV antibodies in human sera.