Epitope mapped
Linear epitope

anti-AAV VP1/VP2 mouse monoclonal, A69, lyophilized, purified

Cat. No: 61057

Available, delivery time 1-7 days

$438.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IP and WB
  • Reacts with AAV2 and AAVDJ
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IP, WB
Purification Affinity chromatography
Reactivity AAV2, AAVDJ
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone A69
Immunogen AAV2 capsids
Concentration 50 µg/ml after reconstitution with 1 ml dist. water

Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:500 (0.1 µg/ml)
Background
A69 reacts with VP1 and VP2 of adeno-associated virus 2 (AAV2) which are highly enriched in the nucleus. Epitope mapping experiments (Wobus et al. 2000) identified aa169 to aa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

References/Publications (3)
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FAQs

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes

AAV9 ELISA Control
Cat. No: PRAAV9-C
  • Consists of fully assembled, empty AAV9 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV8 ELISA Control
Cat. No: PRAAV8-C
  • Consists of fully assembled, empty AAV8 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV6 ELISA Control
Cat. No: PRAAV6-C
  • Consists of fully assembled, empty AAV6 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV5 ELISA Control
Cat. No: PRAAV5-C
  • Consists of fully assembled, empty AAV5 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV3 ELISA Control
Cat. No: PRAAV3-C
  • Consists of fully assembled, empty AAV3 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV2 ELISA Control
Cat. No: PRAAV2-C
  • Consists of fully assembled, empty AAV2 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
AAV1 ELISA Control
Cat. No: PRAAV1-C
  • Consists of fully assembled, empty AAV1 capsids
  • Represents serotype-specific conformational epitopes
  • Reliable positive control for ELISA

$135.00*
OptiPrep™ (Iodixanol)
Cat. No: 1114542
  • Ready-made, sterile and endotoxin-tested solution of iodixanol
  • Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
  • Separation of viable from non-viable cells
  • Also suitable for self-generated gradients
  • Highly effective for purification of viruses and virus particles
  • Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins

$195.00*
anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified
Cat. No: 61056
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with VP1 of AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 and AAVDJ
  • Isotype: IgG2a

$461.00*
anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum
Cat. No: 61084
  • Rabbit polyclonal
  • Suitable for ELISA, ICC/IF, IP and WB
  • Reacts with AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11 and AAV12

$461.00*