anti-S100 Protein rabbit polyclonal, serum

Cat. No: 16100

Available, delivery time 1-7 days

$285.00*

Key Features
  • Rabbit polyclonal
  • Suitable for IHC on frozen and paraffin sections
  • Reacts with bovine and human
Product description
Quantity 250 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Stabilized antiserum
Reactivity Bovine, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen S-100 protein from bovine brain
Formulation Contains 0.09% sodium azide
UniprotID Q0P561 (Bovine),P23297 (Human)
Synonym Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1, S100A1, S100A
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
Background

Reacts with the 21-24 kD (the A and B subunits) of the S-100 protein. S-100A is composed of one alpha- and one beta-chain, whereas S-100B is composed of two beta-chains. S-100 is a brain protein normally present in astrocytes, melanocytes, ependymal cells, glial cells and Schwann cells. Langerhans cells in the skin and interdigitating reticulum cells in the paracortex of lymph nodes also stain positively. S-100 occurs in tumors of the central nervous system, such as Schwannomas, ependymomas, astrogliomas, gliastomas and almost all melanomas.

Positive control: human skin.

References/Publications (1)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
anti-rabbit IgG goat polyclonal, HRP conjugate
Cat. No: 90003
  • HRP conjugate
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with rabbit

$109.00*