OptiPrep™ (Iodixanol)
Available, delivery time 1-7 days
$198.00*
- Ready-made, sterile and endotoxin-tested solution of iodixanol
- Suitable for the isolation of many different cell types from blood and tissue as well as for the isolation of cell organelles and membrane fractions
- Separation of viable from non-viable cells
- Also suitable for self-generated gradients
- Highly effective for purification of viruses and virus particles
- Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins
Product description
| Quantity | 1x250 ml |
|---|---|
| Intended use | Research use only |
| Product description | OptiPrep™ is a ready made, sterile and endotoxin tested solution of Iodixanol, 5,5’-[(2-hydroxy-1-3 propanediyl)-bis(acetylamino)] bis [N,N’- bis(2,3dihydroxypropyl-2,4,6-triiodo-1,3-benzenecarboxamide], designed for the in vitro isolation of biological particles. |
| Density | 1.320 +/- 0.001 g/ml |
| Composition | Iodixanol: 60% (w/v) in water |
| Osmolality | 170 ± 15 mOsm |
| Endotoxin | < 1.0 EU/ml |
| Stability & storage | OptiPrep™ is stable for 3 years provided the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight leads to re- lease of iodine from the molecule. This effect is negligible when working with these solutions on a day to day basis. OptiPrep™ should be stored between 4°C - 24°C. Before use invert the bottles several times to mix the contents. |
Background
OptiPrepTM is a sterile endotoxin tested solution of 60% iodixanol in water with a density of 1.32 g/ml. Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing. Iodixanol is non-ionic, non-toxic to cells and metabolically inert. Iodixanol solutions can be made isoosmotic at all useful densities. Iodixanol solutions have low viscosity and osmolality. Actual endotoxin levels in each batch are usually measured at < 0.13 EU/ml.
The high density of OptiPrepTM facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient or through a simple density barrier.
Improved resolution of cell organelles. Low viscosity, isoosmotic gradients provide rapid and efficient separation of the major organelles in preformed gradients.
OptiPrepTM is the ideal solution for virus purification. Virus purified using OptiPrepTM gradients shows infectivity:particle number ratios at least 100x those from CsCl gradients.
Protocols and references are available for the isolation of cells, macromolecules, subcellular organelles and membranes and virus purification.
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