OptiPrep™ - Iodixanol
Available, delivery time 1-7 days
$198.00*
- Highly effective for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol
Product description
| Quantity | 1x250 ml |
|---|---|
| Intended use | Research use only |
| Product description | OptiPrep™ is a ready made, sterile and endotoxin tested solution of Iodixanol, 5,5’-[(2-hydroxy-1-3 propanediyl)-bis(acetylamino)] bis [N,N’- bis(2,3dihydroxypropyl-2,4,6-triiodo-1,3-benzenecarboxamide], designed for the in vitro isolation of biological particles. |
| Density | 1.320 +/- 0.001 g/ml |
| Composition | Iodixanol: 60% (w/v) in water |
| Osmolality | 170 ± 15 mOsm |
| Endotoxin | < 1.0 EU/ml |
| Stability & storage | OptiPrep™ is stable for 3 years provided the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight leads to release of iodine from the molecule. This effect is negligible when working with these solutions on a day to day basis. OptiPrep™ should be stored between 4°C - 24°C. Before use invert the bottles several times to mix the contents. |
Background
1. Purification of viral particles such as AAV
Benefits of OptiPrepTM for AAV purification:
- Saves time – no need to remove iodixanol for most downstream processes
- Not destructive – causes little or no damage to viral particles
- Greater recovery rate – ten times greater than CsCI gradient alternatives
- Higher infectivity titer –no toxic effects
2. Isolation of full and empty AAV capsids
- Reduce patient risk by minimizing unwanted immune responses
- Maximize your success rate – improved transduction efficiency allows you to use lower titers
- Be ready for clinical trials – focusing on increased safety ensures a smoother transfer
- Manufacture high quality products – by combining with your chromatography manufacturing process
3. Isolation of other biological particles
The low viscosity of the isoosmotic gradient (OptiprepTM) provides a rapid and efficient separation as well as an improved resolution of the major cell organelles.
- Mammalian and non-mammalian cells
- Subcellular organelles
- Plasma membranes and domains
- Membrane vesicles and cytosol
- Organelles from non-mammalian sources
- Plasma lipoproteins
- Proteins and protein complexes
- Plasmid DNA
- Ribonucleoproteins
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