RAP, rat recombinant, 500 µg

Cat. No: 62321

Available, delivery time 1-7 days

$914.00*

Key Features
Product description
Quantity 500 µg
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Source Rat, recombinant (his:RAP:c-myc fusion protein)
Molecular weight 40 kDa
Purity > 95% (determined by SDS gelelectrophoresis)
Reconstitution
When reconstituted with 500 μl distilled water, solution will contain 1 mg/ml rat RAP protein in TBS, pH 7.5, 0.1% BSA, 0.09% NaN3.

Application Protein standard in 1D and 2D SDS gelelectrophoresis, immunoblotting and receptor-binding studies
Background
Protein standard for immunoblotting, immunization, immunoassay and receptor-binding studies. Sequence highly homolog with human RAP.
Rat his:RAP:c-myc fusion protein.
Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (Bajari et al. 2005). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
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