RAP, rat recombinant, 500 µg
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Source||Rat, recombinant (his:RAP:c-myc fusion protein)|
|Molecular weight||40 kDa|
|Purity||> 95% (determined by SDS gelelectrophoresis)|
When reconstituted with 500 μl distilled water, solution will contain 1 mg/ml rat RAP protein in TBS, pH 7.5, 0.1% BSA, 0.09% NaN3.
|Application||Protein standard in 1D and 2D SDS gelelectrophoresis, immunoblotting and receptor-binding studies|
Protein standard for immunoblotting, immunization, immunoassay and receptor-binding studies. Sequence highly homolog with human RAP.
Rat his:RAP:c-myc fusion protein.
Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (Bajari et al. 2005). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
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