Epitope mapped
anti-Butyrophilin mouse monoclonal, 102.43, supernatant
Butyrophilin staining on human lactating breast (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
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Butyrophilin staining on human lactating breast (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
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Key Features
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with bovine and human
- Isotype: IgG2a kappa
Product description
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG2a kappa |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Bovine, Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | 102.43 |
| Immunogen | Butyrophilin purified from bovine milk fat globule membrane |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | P18892 (Bovine),Q13410 (Human) |
| Synonym | Butyrophilin subfamily 1 member A1, BT, BTN1A1, BTN |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | Ready-to-use |
| Western Blot (WB) | Assay dependent |
Background
The mab reacts specifically with butyrophilin, a unique 67 kD polypeptide present in lactating mammary gland epithelium and in the milk fat globule membrane (MFGM).
No cross-reaction with other MFGM proteins. The epitope has been localized on the N-terminal extracellular part of the protein, close to the transmembrane region.
Butyrophilins have recently been described as a new family of immunoregulators, similar to the co-stimulatory and co-inhibitory family of B7 molecules (Arnett & Viney, 2014).
Polypeptide reacting:Butyrophilin, 67 kD polypeptide; present in the apical membrane of lactating mammary epithelium and in MFGM.
Reactivity on cultured cell lines: Transfected cell line BMGE with butyrophilin cDNA.
Positive control: Milk fat globule membrane (MFGM).
Arnett HA & Viney JL: Immune modulation by butyrophilins. Nature Review Immunology 14, 559-569 (2014)
References/Publications (1)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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