anti-CD174 mouse monoclonal, EBS-CD-049, purified

Cat. No: 691616

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-049
Immunogen Neuraminidase-treated live T-47D breast carcinoma cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q11126 (Bovine), P21217 (Human)
Synonym 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, EC, Alpha-3-fucosyltransferase FUT3, EC 2.4.1.-, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, FT3B, LE
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Flow Cytometry (FACS) Assay dependent
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)

EBS-CD-049 recognizes LeY (CD174) and to some extent Leb. EBS-CD-049 displays no cross-reactivity with blood group antigens A, B, Lea, LeX, Sialyl-Lea, Sialyl-LeX, nor with H type 1, type 2, or type 3, nor with 74 other synthetic oligo- and mono-saccharides tested (minimal reactivity exists to Globo-H). In addition, a protein conformational epitope on human and bovine histone H1 is detected (mimicry). Histones H2A, H2B, H3 and H4 are not detected.

Positive control: breast or colorectal cancer.

Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.