anti-CD71 mouse monoclonal, EBS-CD-040, purified
- Mouse monoclonal
- Suitable for FACS, ICC/IF, IHC and WB
- Reacts with human
- Isotype: IgG1 kappa
|Quantity||1 ml (100 µg/ml)|
|Application||FACS, ICC/IF, IHC, WB|
|Intended use||Research use only|
|Formulation||PBS with 0.02% sodium azide|
|Synonym||Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, CD antigen CD71 [Cleaved into: Transferrin receptor protein 1, serum form, sTfR], TFRC|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:100-1:200 (0.5-1.0 µg/ml)|
|Immunohistochemistry (IHC) - frozen||1:50-1:100 (1-2 µg/ml)|
|Flow Cytometry (FACS)||0.5-1.0 µg/million cells in 0.1 ml|
|Western Blot (WB)||1:50-1:100 (1-2 µg/ml)|
The transferrin receptor is a type II membrane glycoprotein existing as a homodimer of 180-190 kDa with interchain disculphide bonding. The ligand is the serum iron transport protein transferrin. CD71 is expressed weakly on all resting leucocytes, but is upregulated on all cells upon activation, reflecting the iron dependence of proliferation. In other tissues CD71 is expressed on most dividing cells, but also strongly on brain endothelium and alveolar macrophage. CD71 expression can reflect clinical behaviour or response to therapy in a number of malignancies including leukemia, lymphoma and breast cancer.
Positive control: CCRF-CEM, Jurkat, K-562, human placenta, breast carcinoma.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.