Epitope mapped
Disease related

anti-CD81 mouse monoclonal, 1.3.3.22, purified

Cat. No: 691605

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone 1.3.3.22
Immunogen B-cell line derived from a Burkitt lymphoma
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q969P0 (Human)
Synonym Immunoglobulin superfamily member 8, IgSF8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, Prostaglandin regulatory-like protein, PGRL, CD antigen CD316, IGSF8, CD81P3, EWI2, KCT4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

1.3.3.22 reacts with human CD81 (TAPA-1), a 24 kDa member of the TM4 superfamily of proteins with four transmembrane domains. MAbs to CD81 have been shown to have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 can associate with CD37 and/or CD53, or on the surface of B-cells with CD19, CD21 and/or MHC class II molecules. 1.3.3.22 was clustered at the VIth WLDA.

Positive control: ramos cells, human tonsil.

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