anti-I-CAM mouse monoclonal, 101-1D2, supernatant
- Mouse monoclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated from human leucocytes|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD antigen CD50, ICAM3|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:10|
|Immunohistochemistry (IHC) - paraffin||1:10 (microwave treatment recommended)|
The antibody reacts with the cellular adhesion molecule CD50 (ICAM-3). CD50 is a single chain polypeptide with a MW of 120 kD. The protein is heavily glycosylated and resistant to treatment with phosphatidylinositol phospholipase C, and therefore probably not PI-anchored to the membrane. The antibody was provisionally clustered as CDw50 in Vienna at the IV and as CD50 in Boston at the V international workshop on human leucocyte differentiation antigens.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.