Highly published

anti-Keratin K8/K18 guinea pig polyclonal, serum

Cat. No: GP11

Prices excl. tax

Available, delivery time 1-7 days

$375.00*

Datasheet

Key Features

Stabilized antiserum
Guinea pig polyclonal
Suitable for ELISA, IHC and WB
Reacts with bovine, chicken, human, mouse, rat and hamster

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	ELISA, IHC, WB
Purification	Stabilized antiserum
Reactivity	Bovine, Chicken, Hamster, Human, Mouse, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Formulation	Contains 0.09% sodium azide
UniprotID	P05786 (Bovine), A0A1L1RIW5 (Chicken), P05787 (Human), P11679 (Mouse), Q10758 (Rat)
Synonym	Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100
Immunohistochemistry (IHC) - paraffin	1:50-1:100 (microwave treatment recommended)
ELISA	Assay dependent
Western Blot (WB)	1:1,500-1:2,500

Background

Reactive polypeptide (specificity): Mr 52,500 and 45,500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors specifically detected: all adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma.

References/Publications (33)

Publication

Species

Application

Publication Kim, M. et al. Plakophilin 1 deficiency in prostatic tumours is correlated with immune cell recruitment and controls the up-regulation of cytokine expression post-transcriptionally. FEBS J. (2022).

Species human

Application IHC-IF (paraffin)

Publication Kakehashi, A. et al. Canopy homolog 2 as a novel molecular target in hepatocarcinogenesis. Cancers (Basel). 13, 3613 (2021).

Species mouse

Application IHC

Publication Komai, M. et al. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat. Vet.Sci. 6, (2019)

Species rat

Application IHC (paraffin)

Publication Hollern, D. et al. A mouse model featuring tissue-specific deletion of p53 and Brca1 gives rise to mammary tumors with genomic and transcriptomic similarities to human basal-like breast cancer. Breast.Cancer.Res.Treat. 174, 143-155 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Linde, N. et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat.Commun. 9, 21 (2018).

Species mouse

Application IHC (frozen)

Publication Williams, J. et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J.Cell.Sci. 131, (2018).

Species mouse

Application ICC-IF

Publication Sarrach, S. et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci.Rep. 8, 1801 (2018).

Species mouse

Application IHC (frozen)

Publication Song, Y. et al. RB inactivation in keratin 18 positive thymic epithelial cells promotes non-cell autonomous T cell hyperproliferation in genetically engineered mice. PLoS One 12, e0171510 (2017)

Species mouse

Application ICC-IF

Publication Kakehashi, A. et al. Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital. Oxid.Med.Cell.Longev. 2017, 8541064 (2017).

Species mouse

Application IHC (paraffin)

Publication Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015).

Species mouse

Application IHC (free floating sections)

Publication Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015).

Species human

Application IHC (paraffin)

Publication Russell, R. et al. Loss of ATM accelerates pancreatic cancer formation and epithelial-mesenchymal transition. Nat. Commun. 6, 1–16 (2015).

Species mouse

Application IHC

Publication Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013).

Species mouse

Application IHC (paraffin)

Publication Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013).

Species mouse

Application IHC (paraffin),ICC-IF

Publication König, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).

Species mouse

Application IHC (paraffin)

Publication Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012).

Species mouse

Application IHC (paraffin)

Publication Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012).

Species mouse

Application WB,IHC (paraffin),ICC-IF

Publication Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012).

Species mouse

Application WB

Publication Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/âˆ’ Mice. Am. J. Pathol. 176, 1421–1432 (2010).

Species mouse

Application IHC (paraffin)

Publication Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010).

Species human

Application IHC (frozen)

Publication Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010).

Species mouse

Application IHC (paraffin)

Publication Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009).

Species rat

Application IHC (free floating sections)

Publication Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009).

Species human

Application IHC (frozen)

Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008).

Species human

Application IHC (frozen)

Publication Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008).

Species mouse

Application IHC (paraffin)

Publication Hüsemann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008).

Species mouse

Application ICC-IF

Publication Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007).

Species human

Application IHC (paraffin)

Publication Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006).

Species mouse

Application WB,IHC (paraffin)

Publication Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005).

Species human

Application IHC (frozen)

Publication Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005).

Species mouse

Application IHC (paraffin)

Publication Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002).

Species mouse

Application WB

Publication Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67–79 (1989).

Species human

Application WB,ICC-IF

Publication Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987).

Species human

Application IHC (frozen),IEM

Downloads

File

Category

Size

Filetype

File IHC guinea pig antibody

Category Protocol

Size 167.92 KB

Filetype pdf

File WB protocol

Category Protocol

Size 210.69 KB

Filetype pdf

Datasheet

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FAQs

PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
Use the antibody in a higher dilution, but prolong incubation time and exposure time.
Always use a fresh aliquot of the antibody.
Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
Include an additional washing step.
You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
Always use a fresh aliquot of secondary antibody.
In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Progen Support

For scientific and customer support, you can reach us by phone or email.

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anti-Keratin K8/K18 guinea pig polyclonal, serum

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