anti-Lamin A + C mouse monoclonal, R27, supernatant
anti-Lamin A + C mouse monoclonal, R27, supernatant
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anti-Lamin A + C mouse monoclonal, R27, supernatant
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Key Features
- Mouse monoclonal
- Suitable for ICC/IF and WB
- Reacts with bovine, human, mouse, trout, xenopus and zebrafish
- Isotype: IgM
Product description
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgM |
| Conjugate | Unconjugated |
| Application | ICC/IF, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Bovine, Human, Mouse, Trout, Xenopus, Zebrafish |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | R27 |
| Immunogen | Nuclear pore complex-lamina fraction of bovine liver |
| Formulation | Contains 0.09% sodium azide |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Ready-to-use |
| Western Blot (WB) | Assay dependent |
Background
The monoclonal antibody decorates the lamina, i.e. the nucleoplasmic layer coating the nuclear envelope.
Mutations in lamin A have been shown to cause Hutchinson-Gilford progeria syndrome in human and mouse and other laminopathies (for review see Burke & Stewart, 2002).
Polypeptides reacting: Lamin isotypes A and C (Mr 60 - 75 kDa); meiotic lamin C2
Reactivity in cultured cell lines: RV-SMC (rat vascular smooth muscle cells)
References/Publications (2)
Publication
Species
Application
Species
rat
Application
ICC-IF
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- For detection of lamin Type A in fish an enriched sample (e.g. nuclear fraction) should be used and loaded in large quantity onto the gel to obtain enough antigen.
- For mammalian cells it is recommended to make a nuclear membrane extract from a minimum of 106 cells.
- The antibody is an IgM subtype with low affinity, therefore the dilution should not be too high.
- The secondary system should be specific for IgM-type antibodies.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
Hepatocytes
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
- Tissue (5 µm sections) fixation 5 min in methanol (-20°C) and 5 min in acetone (-20°C).
- Air drying.
- Optional step: Dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 5 min at room temperature (enhances epitope accessibility of many cytoskeletal antigens).
- Air drying of the section.
- Block with the serum of the species in which the secondary antibody was raised for 30 min at RT.
- Incubation with undiluted 1st antibody 1 h at RT or overnight at 4°C in moist chamber.
- Wash 3x with PBS.
- Incubation with appropriate secondary antibody (with fluorescence dye), 30-60 min at RT.
- Wash 3x with PBS.
- Immerse shortly into ethanol.
- Let air dry.
- Cover with mounting medium for microscopy.
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