anti-MADER (NAB2) mouse monoclonal, EBS-T-005, purified
- Mouse monoclonal
- Suitable for IHC
- Reacts with human
- Isotype: IgG1 kappa
|Quantity||1 ml (100 µg/ml)|
|Intended use||Research use only|
|Immunogen||Partial recombinant NAB2|
|Formulation||PBS with 0.02% sodium azide|
|Synonym||NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER, NAB2, MADER|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:50-1:100 (1-2 µg/ml)|
EBS-T-005 reacts with MADER or Melanoma-Associated Delayed Early Response protein, encoded by the NAB2 gene, belonging to the family of NGFI-A binding proteins. They modulate transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can form homo- or hetero-multimers with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains.
Positive control: melanoma.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.