anti-MHC I ABC mouse monoclonal, Bra23/9, purified

Cat. No: 691540

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, IHC and IP
  • Reacts with human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ELISA, FACS, IHC, IP
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone Bra23/9
Immunogen Non-T, non-B human acute lymphoblastic leukemia REH6 cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Immunoprecipitation (IP) Assay dependent
Background

Bra23/9 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells and platelets and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Positive control: THP-1 and Jurkat cells. Tonsil or lymph node.

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