Highly published

anti-Nephrin guinea pig polyclonal, serum

Cat. No: GP-N2

Available, delivery time 1-7 days

$312.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide of mouse Nephrin (intracellular domain, aa1243-1256, EPGSLPFELRGHLV)
Formulation Contains 0.09% sodium azide
UniprotID O60500 (Human), Q9QZS7 (Mouse)
Synonym Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:500
Background

The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE).
Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization: the antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Tested cultured cell lines: PCL (podocyte cell line), M-1 (cortical collecting duct cells).

References/Publications (68)
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