anti-SALL4 mouse monoclonal, IHC659, purified
Testicular cancer
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Testicular cancer
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Key Features
- Mouse monoclonal
- Suitable for IHC
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Affinity chromatography |
| Reactivity | Human |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | IHC659 |
| Immunogen | Recombinant Human SALL4 |
| Formulation | Tris pH 7.3-7.7 with 1% BSA and 0.09% sodium azide |
| UniprotID | Q9UJQ4 (Human) |
| Synonym | Sal-like protein 4, Zinc finger protein 797, Zinc finger protein SALL4, SALL4, ZNF797 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - paraffin | 1:50-1:200 (microwave treatment recommended) |
Background
Sal-Like Protein 4 (SALL4) is a zinc finger transcription factor found in germ cells and human blood progenitor
cells, with functional involvement in modulating Oct-4 to maintain embryonic stem cell pluripotency. SALL4
is a useful marker for acute myeloid leukemia, B-cell acute lymphocytic leukemia, intratubular germ cell
neoplasia, seminomas/dysgerminomas, and yolk sac tumours (both pediatric and postpubertal). Anti-SALL4
is used to detect embryonal carcinomas, hepatocellular carcinoma (HCC), gliomas, ovarian primitive germcell
tumours, choriocarcinomas, spermatogonia, teratoma, gastric cancer, breast cancer, and lung cancer.
Expression of SALL4 is often associated with poor prognosis in HCC, and with metastasis in endometrial
cancer, colorectal carcinoma, and esophageal squamous cell carcinoma.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.