anti-p21 Protein mouse monoclonal, WA-1, supernatant
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Rec. WAF-1/p21 protein|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
|Immunohistochemistry (IHC) - paraffin||1:5-1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
p21 is a nuclear 21 kD protein, a product of the WAF-1/CIP1 gene. It is a constituent of a large complex of nuclear proteins, including cyclins, cyclin dependent kinases and PCNA. After activation by the wild type tumor suppressor protein p53, WAF-1 acts as an inhibitor of the cell cycle during G1 phase. Normal cells generally display a rather intense nuclear p21 expression. In several malignancies (e.g. breast cancer, bladder cancer, head and neck scc) it has been shown that the presence of mutations in the p53 gene are of prognostic significance. Demonstration of low expression of p21 in a p53 positive tumor will increase the likelihood of the presence of a p53 mutation. p21 immunostaining is therefore most often used in combination with p53 to confirm the presence of a p53 mutation. Overexpression of p53 with normal intensity of p21 argues against a p53 mutation, while p53 overexpression in combination of low or absent p21 expression is consistent with a p53 mutation.
Positive control: Tonsil.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.