anti-Complement C5b neo-Epitope mouse monoclonal, HCC5b.1, lyophilized, purified

Cat. No: 61022

Available, delivery time 1-7 days

$251.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine, goat, human, pig and primates
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Goat, Human, Pig, Primates
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone HCC5b.1(neo)
Immunogen Activated human complement component C5
Concentration 50 µg/ml after reconstitution with 1ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

HCC 5b.1(neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5!
Polypeptide reacting: Mr 200 kDa polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 kDa; after C5b activation, an Mr 60 kDa cleavage product also reacts (weakly).
Disorders specifically detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC5.1.

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