anti-Complement C3b beta mouse monoclonal, H11, lyophilized, purified

Cat. No: 61020

Available, delivery time 1-7 days

$264.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC, WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone H11
Immunogen Human complement component C3
Concentration 50 µg/ml after reconstitution with 1ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) Assay dependent
Background

H11 allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d; inhibits hemolytic function of C3. H11 specifically reacts with C3b beta-chain.

Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-alpha (H206), and negative control anti C3a (H13). Activated, deposited C3b bears 3b-alpha and -beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H13.

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