Disease related

anti-Desmoglein 1 mouse monoclonal, Dsg1-P23, supernatant concentrate

Supernatant

Supernatant concentrate

Cat. No: 652110

Available, delivery time 1-7 days

$510.00*

Key Features
  • Hybridoma cell culture supernatant concentrate
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 500 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant concentrate
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Dsg1-P23
Immunogen Recombinant human polypeptide Desmoglein 1 (extra-cellular part EII, EIII and EIV)
Formulation Contains 0.09% sodium azide
UniprotID Q02413 (Human)
Synonym Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, DSG1, CDHF4
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:1,000 (treatment with 0.05-0.02% Triton X-100 is recommended, see Schäfer, S. et al. 1996 for protocol)
Immunohistochemistry (IHC) - paraffin 1:1,000 (preincubation with 0.001% trypsin, addition of 2% milk powder to the incubation media, and microwave treatment is recommended)
Western Blot (WB) Assay dependent
Background
In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kDa polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.
References/Publications (2)
Publication
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