Epitope mapped
Frequently used
Diagnostic marker

anti-Actin mouse monoclonal, 2G2, supernatant

Cat. No: 651132

Available, delivery time 1-7 days

$293.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, plant and rabbit
  • Isotype: IgM
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Purification Hybridoma cell culture supernatant
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 2G2
Immunogen Profilin actin complex from calf thymus
Formulation Contains 0.09% sodium azide
UniprotID Q3B7N2 (Bovine), P05094 (Chicken), P12814 (Human), A0A5F9CV24 (Rabbit)
Synonym Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent (IF only after methanol fixation)
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (protease treatment recommended)
Western Blot (WB) Assay dependent
Background

Specific for skeletal and non-muscle actin isoforms (43 kDa). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).

Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.

Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)

References/Publications (9)
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Actin, rabbit, 250 µg
Cat. No: 62001
Quantity: 250 µg
  • Purified rabbit actin
  • Protein standard

Variants from $123.00*
$185.00*
anti-Actin mouse monoclonal, 647, supernatant
Cat. No: 10601
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with chicken, human, mouse and rat
  • Isotype: IgM

$306.00*