anti-Actin mouse monoclonal, 647, supernatant
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with chicken, human, mouse and rat
- Isotype: IgM
|Application||ICC/IF, IHC, WB|
|Purification||Hybridoma cell culture supernatant|
|Reactivity||Chicken, Human, Mouse, Rat|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Antigen isolated from chicken skeletal muscle cells|
|Formulation||Contains 0.09% sodium azide|
|UniprotID||P05094 (Chicken),P12814 (Human),Q7TPR4 (Mouse),Q9Z1P2 (Rat)|
|Synonym||Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:5 - 1:10|
|Immunohistochemistry (IHC) - paraffin||1:5 - 1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Mab 647 is useful for studying the intracellular distribution and structure of actin in the cytoskeletal system. In immunoblotting one single band is reactive corresponding to actin.
Positive control: Muscle or myofibrils.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.