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Phage Display

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Protocols

  1. Hust M., Frenzel, A., Tomszak, F, Kügler, J & Dübel, S. (2014) Antibody Phage Display. In: Dübel, S. & Reichert, J.M. (eds.) Handbook of Therapeutic Antibodies, 2nd ed. Wiley-VCH, Weinheim, ISBN 978-3-527-32937-3 p. 43-76
  2. Hust M., Frenzel, A., Tomszak, F, Kügler, J & Dübel, S. (2014) Antibody Hust, M., Frenzel, A., Schirmann, T. and Dübel, S. (2014) Selection of recombinant antibodies from antibody gene libraries. Methods Mol Biol. 1101, 305-320.2
  3. Hust, M., Frenzel, A., Meyer, T., Schirmann, T. and Dübel, S. (2012). Construction of human naive antibody gene libraries. In: Antibody Engineering: Methods and Protocols, Ed: Chames, P., Meth Mol Biol 907, 85-107
  4. Breitling, F., Broders, O., Helmsing, S., Hust, M & Dübel, S (2010) Improving phage display throughput by using Hyperphage, miniaturised titration and pVIII (g8p) ELISA. In: Antibody Engineering (2nd ed) Springer Protocols. Vol. 1, ISBN 978-3-642-01143-6, p. 197-206
  5. Broders, O., Breitling, F., and Dübel, S. (2003). Hyperphage - Improving Antibody Presentation in Phage Display. Methods Mol Biol 205, 295-302.

Original papers on the succesful use of the hyperphage system

  1. Goffin L, Fagagnini S, Vicari A, Mamie C, Melhem H, Weder B, Lutz C, Lang S, Scharl M, Rogler G, Chvatchko Y, Hausmann M.Anti-MMP-9 Antibody: A Promising Therapeutic Strategy for Treatment of Inflammatory Bowel Disease Complications with Fibrosis.Inflamm Bowel Dis. 2016 Sep;22(9):2041-57.
  2. Mendonça M, Moreira GMSG, Conceição FR, Hust M, Mendonça KS, Moreira ÂN, et al. (2016) Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. PLoS ONE 11(8): e0160544.
  3. Zantow, J., Just. S., Lagkouvardos, I., Kisling. S., Dübel, S., Lepage. P., Clave, T. & Hust, M. (2016) Mining gut microbiome oligopeptides by functional metaproteome display. Sci. Rep. 6:34337.
  4. Connor DO, Zantow J, Hust M, Bier FF, von Nickisch-Rosenegk M. Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display. PLoS One. 2016 Feb 9;11(2):e0148986.
  5. Derman, Y., Selby, K., Miethe, S., Frenzel, A., Liu, Y., Rasetti-Escargueil, C., Avril, A., Pelat, T., Urbain, R., Fontayne, A., Thullier, P., Sesardic, D., Lindström, M., Hust, M. and Korkeala, H. (2016). Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody. Toxins 8: 25
  6. Rohrbeck, A., Fühner, V., Schröder, A., Hagemann, S., Vu, X.-H., Berndt, S., Hust, M., Pich, A. and Just, I. (2016). Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody. Toxins 8:100
  7. Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). Development of Germline-Humanized Antibodies Neutralizing Botulinum Neurotoxin A and B. PLoS ONE 11: e0161446
  8. Blokzijl, A., Zieba, A., Hust, M., Schirrmann, T., Helmsing, S., Grannas, K., Hertz, E., Moren, A., Chen, L., Soderberg,, O., Moustakas, A., Dübel, S., & Landegren, U. (2016) Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Mol Cell Proteomics 15, 1848-1856
  9. Kibat, J, Schirrmann. T., Knape, M.J.,, Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. & Hoheisel, J.D. (2016) Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. N. Biotech. 33, 574-581
  10. Rasetti-Escargueil, C., Avril, A., Tierney, R., Bak, N., Miethe, S., Chahboun, S., Mazuet, C., Popoff, M., Thullier, P., Hust, M., Pelat, T. and Sesardic, D. (2015). Development of human-like scFv-Fc neutralising Botulinum toxin type B. mAbs 6: 1161-1177
  11. Miethe, S., Rasetti-Escargueil, C., Avril, A., Liu, Y., Chahboun, S., Korkeala, H., Mazuet, C., Popoff, M.-R., Pelat, T., Thullier. P., Sesardic, D. and Hust, M. (2015). Development of human-like scFv-Fc neutralizing botulinum neurotoxin E. PLoS ONE 10: e0139905
  12. Becker, M., Felsberger, A., Frenzel, A., Shattuck, W.M.C, Dyer, M., Kügler, J., Zantow, J., Mather, T. and Hust, M. (2015). Application of M13 Phage Display for Identifying Immunogenic Proteins from Tick (Ixodes scapularis) Saliva. BMC Biotechnology 15: 43
  13. Avril, A., Miethe, S., Popoff, M., Mazuet, C., Chahboun, S., Rassetti-Escargueil, C., Sesardic, D., Thullier, P., Hust, M. and Pelat, T. (2015). Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain. BMC Biotechnology 15: 86
  14. Droste P., Frenzel A., Steinwand, M., Pelat, T., Thullier, P., Hust, M., Lashuel, H. and Dübel, S. (2015) Structural differences of amyloid-β fibrils revealed by antibodies from phage display. BMC Biotechnol.15:57
  15. Zehner, M., Marschall, A.L.J., Erik Bos, E., Schloetel, J.-G., Kreer, C., Fehrenschild, D., Limmer, A., Ossendorp, F., Lang, T., Koster, A.J., Dübel, S., Burgdorf, S. (2015) Endosomal Sec61 mediates antigen translocation in the cytosol for cross-presentation. Immunity 42:850-863
  16. Kügler, J., Wilke. S., Doris, M., Tomszak, F., Frenzel, A., Schirrmann, T., Dübel, S., Garritsen, H., Hock. B., Toleikis, L., Schütte, M. and Hust. M. (2015) Generation and analysis of the improved human HAL9/10 antibody phage display libraries. BMC Biotechnol. 15:10
  17. Fuchs, M., Kämpfer, S., Spallek, R., Oehlmann, W., Prilop, W., Frank, R., Dübel, S., Singh, M. & Hust, M. (2014) Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B. BMC Biotechnol. 14:68
  18. Hülseweh, B., Rülker, T., Pelat, T., Langermann, C., Frenzel, A., Schirrmann, T., Dübel, S., Thullier, P. & Hust, M., (2014) Human-like antibodies neutralizing Western Equine Encephalitis Virus. mAbs 6, 717 - 726
  19. Trott, M., Weiß, S., Antoni, S., Koch, J., von Briesen, H., Hust, M. and Dietrich, U. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibody. PLoS ONE 9: e97478
  20. Miethe, S. , Pelat, T., Avril, A., Popoff, M., Molgo, J., Rasetti Escargueil, C., Fontayne, A., Korkeala, H., Thullier, P., Sesardic, D. and Hust, M. (2014). Development of recombinant antibodies for biodefense using phage display. STO Scientific Publications, STO-MP-HFM239-16
  21. Miethe, S., Rasetti-Escargueil, C., Liu, Y., Chahboun, S., Pelat, T., Avril, A., Frenzel, A., Schirrmann, T., Thullier, P., Sesardic, D. and Hust, M. (2014). Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library. mAbs 6: 446-459
  22. Schirrmann, T. Frenzel, A., Linden, L., Stelte-Ludwig, B., Willuda, J., Harrenga, A., Dübel, S., Müller-Tiemann, B. & Trautwein, M. (2014) Evaluation of human pancreatic RNase as effector molecule in a therapeutic antibody platform. mAbs, J6(2)
  23. Steinwand, M., Droste, P., Frenzel, A., Hust, M., Dübel, S. and Schirrmann, T. (2013) The influence of antibody fragment format on phage display based affinity maturation of IgG. mAbs, 6, 204-218
  24. Hust Zhou, M., Meyer, T., Koch, S., Koch, J., Brill, B., von Briesen, H., Benito, J.M., Soriano, V., Haberl, A., Bickel, M., Dübel, S., Hust, M., and Dietrich, U. (2013) Identification of a new epitope for HIV-neutralizing antibodies in the gp41 membrane proximal external region by an Env-tailored phage display library. Eur J immunol, 43, 499-509
  25. Meyer, T., Schirrmann, T., Frenzel, A., Miethe, S., Stratmann-Selke, J., Gerlach, G.F., Strutzberg-Minder, K., Dübel, S., and Hust, M. (2012) Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display. BMC Biotechnol, 12:29
  26. Wezler, X., Hust, M., Helmsing, S., Schirrmann, T. & Dübel, S. (2012) Human antibodies targeting CD30+ lymphomas. Hum. Antibod. 21:13-28
  27. Rülker, T., Voß, L., Thullier, P., O’ Brien, L.M., Pelat, T., Perkins, S.D., Langermann, C., Schirrmann, T., Dübel, S., Marschall, H.-J., Hust, M. & Hülseweh, B. (2012) Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo. PLoS ONE, 7(5): e37242. doi:10.1371/journal.pone.0037242
  28. Zhang, C., Helmsing, S., Schirrmann, T., Marschall, A.L.J., Zagrebelsky, M., Hust, M., Korte, M & Dübel, S. (2012) Suppression of p75 neurotrophin receptor surface expression with intrabodies influences Bcl-xL mRNA expression and neurite outgrowth in PC12 cells. PLoS ONE 7(1): e30684. doi:10.1371/journal.pone.0030684
  29. Colwill, K., Renewable Protein Binder Working Group & Gräslund, S., (2011) A roadmap to generate renewable protein binders to the human proteome. Nature Meth. 8, 551–558
  30. Hust, M., Meyer, T., Voedisch, B., Rülker, T., Thie, H., Schütte, M., Helmsing, S., Meier, D., Schirrmann, T., Dübel, S. (2011) A human scFv antibody generation pipeline for proteome research, J. Biotechnol. 152,159-170
  31. Meyer, T., Stratmann-Selke, J., Meens, J., Schirrmann, T., Gerlach, G.F., Frank, R., Dübel, S., Strutzberg-Minder, K., Hust, M. (2010) Isolation of OmpD specific scFv fragments for the diagnosis of Salmonella Typhimurium. Vet. Microbiol. 147, 162-169
  32. Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Nilsson, P., Gräslund, S., Structural Genomics Consortium, Colwill, K., Hust M., and Dübel, S. (2010) Towards Proteome Scale Antibody Selections using Phage Display. N. Biotec, 27, 118-128
  33. Kibat, J, Schirrmann. T., Knape, M.J.,, Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. & Hoheisel, J.D. (2016) Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. N. Biotech. 33, 574-581
  34. Pelat, T., Hust, M., Hale, M., Lefranc, M.P., Dübel, S. & Thullier, P. (2009) Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. BMC Biotechnol., 9:60
  35. Naseem, S., Meens, J., Jores, J., Heller, M., Dübel, S., Hust, M. and Gerlach, G.F.. (2010) Phage display-based identification and potential diagnostic application of novel antigens from Mycoplasma mycoides subsp. mycoides small colony type. Veterinary Microbiology 142, 285-292
  36. Kirsch, M.I., Hülseweh, B., Nacke, C., Rülker, T., Schirrmann, T., Marschall, H.-J., Hust, M. and Dübel, S. (2008). Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV). BMC-Biotechnol. 8, 66
  37. Kügler, J., Nieswandt, S., Gerlach, G.F., Meens, J., Schirrmann, T. and Hust, M. (2008). Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display. Applied Microbiology and Biotechnology 80, 447- 458
  38. Thie H, Schirrmann T, Paschke M, Dübel S, Hust M. (2008) SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. N Biotechnol. 5, 49-54
  39. Pelat, T., Hust, M, Laffly, E., Condemine, F., Bottex, C., Vidal, D., Lefranc, M.P., Dübel, S., Thullier, P. (2007) A high affinity, human like antibody fragment (scFv) neutralising lethal factor (LF) of Bacillus anthracis by inhibiting the PA-LF complex formation. Antimicrob agents and chemother 51, 2758-2764
  40. Soltes G, Hust M, Ng KKY, Bansal A, Field J, Stewart DIH, Dübel S, Cha S, Wiersma E (2007) On the influence of vector design on antibody phage display. J Biotech 127, 626-637
  41. Hust, M., Meysing, M., Schirrmann, T., Selke, M., Meens, J., Gerlach, G.F. & Dübel, S. (2006) Enrichment of open reading frames presented on bacteriophage M13 using Hyperphage. Bio/Techniques, 41, 335-342
  42. Kirsch, M., Zaman, M., Meier, D., Dübel, S. and Hust, M. (2005) Parameters affecting the display of antibodies on phage. J immunol Meth 301, 173-85
  43. Rondot, S., Koch, J., Breitling, F., and Dübel, S. (2001). A helperphage to improve single chain antibody presentation in phage display. Nature Biotechnol. 19, 75-78

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