LymphoPrep™ (6x500 ml)

Cat. No: 1858-6 (previous 1114547)

Available, delivery time 1-7 days

$452.00*

Key Features
  • Ready-made, sterile and endotoxin tested solution
  • Highly effective for the purification of human mononuclear cells (monocytes and lymphocytes) from diluted blood
  • One order unit contains 6x500 ml bottles of Lymphoprep™
Product description
Quantity 6x500 ml
Intended use Research use only
Product description Lymphoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of pure lymphocyte suspensions. The solution contains sodium diatrizoate and polysaccharide.
Density 1.077 ± 0.001 g/ml
Composition 9.1% (w/v) Sodium Diatrizoate, 5.7% (w/v) Polysaccharide
Osmolality 290 ± 15 mOsm
Endotoxin < 1.0 EU/ml
Stability & storage Lymphoprep™ is stable for 3 years provided the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight leads to release of iodine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day to day basis. Lymphoprep™ should be stored at +4°C to +30°C.
Background

A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Boyum in 1968. For more than 35 years a commercial medium known as LymphoprepTM has been widely used for isolating these cells.

Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.

The described method is rapid, simple and reliable and gives excellent results with blood samples from normal individuals and patients.

To obtain the maximum yield it is important that the blood sample is diluted 1:1 with physiological saline before being applied to the gradient.

The contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total cell number.

Some immature PMNs may band with the lymphocytes during intense immunosuppressive therapy. When heparinised blood is used, it is essential to remove most of the platelets, in order to avoid inhibition in the cytotoxicity test.

LymphoprepTM can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera and immunological research. Thorsby and Brattelie used this technique with only slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.

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LymphoPrep™ (6x500 ml)
Cat. No: 1858-6 (previous 1114547)
Quantity: 6x500 ml
  • Ready-made, sterile and endotoxin tested solution
  • Highly effective for the purification of human mononuclear cells (monocytes and lymphocytes) from diluted blood
  • One order unit contains 6x500 ml bottles of Lymphoprep™

Variants from $81.00*
$452.00*
PolymorphPrep™
Cat. No: 1895 (previous 1114683)
  • Ready-made, sterile and endotoxin tested solution of sodium diatrizoate and polysaccharide
  • Suitable for the isolation of human polymorphonuclear leucocytes (granulocytes) from whole undiluted blood

Please note: The Cat. No. of PolymorphPrep™ changes from 1114683 to 1895 but the product itself has not been changed in any way.
Therefore it is possible to receive the ordered products with Cat. No. 1114683 or 1895.

$136.00*
OptiPrep™ - Iodixanol
Cat. No: 1893 (previous 1114542)
  • Highly effective for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

$198.00*
Nycodenz® - Iohexol
Cat. No: 18003 (previous 1002424)
  • Effective for purification of viruses and virus particles
  • Suitable for the isolation of different cell types and organelles
  • Convenient for the separation of viable from non-viable cells
  • Non-ionic, non-toxic and highly soluble in water

$569.00*