anti-Collagen Type IV mouse monoclonal, 1042.0, supernatant
- Mouse monoclonal
- Suitable for IHC
- Reacts with human
- Isotype: IgG2b
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated from human placenta|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Collagen alpha-1(IV chain [Cleaved into: Arresten], COL4A1|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:10–1:20|
|Immunohistochemistry (IHC) - paraffin||1:10–1:20 (protease (pepsin) treatment and/or microwave treatment recommended)|
Antibody 1042 is reactive with human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology.
No crossreactivity with other collagens.
Positive control: Skin, kidney.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.