anti-Desmoglein 4 guinea pig polyclonal, serum
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- Stabilized antiserum
- Guinea pig polyclonal
- Suitable for IHC
- Reacts with human, mouse and rat
Product description
| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Recombinant peptide (extracellular repeat domain E4; aa 472-590) of human Desmoglein 4 |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | Q86SJ6 (Human), Q7TMD7 (Mouse), Q6W3B0 (Rat) |
| Synonym | Desmoglein-4, Cadherin family member 13, DSG4, CDHF13 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100-1:200 (microwave treatment recommended) |
Background
Antibody recognizes desmoglein 4 present in the hair cortex, beginning at the height of the apex of the dermal papilla and cuticle (inner root sheath) from the upper differentiated part.
Also positive staining is found in the stratum granulosum of epidermis.
Polypeptide reacting: Desmoglein 4.
References/Publications (1)
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FAQs
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
- Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).
- Air-drying of the section.
- Block with the serum of the species in which the secondary antibody was raised for 30 min.
- Incubation with 1st antibody 1 h at RT in moist chamber.
- Wash 3x with PBS.
- Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
- Wash 3x with PBS.
- Immerse shortly into ethanol.
- Let air dry.
- Cover with mounting medium.
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