Disease related

anti-Drebrin mouse monoclonal, MX823, supernatant

Supernatant

Ascites fluid

Cat. No: 651128

Available, delivery time 1-7 days

$245.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC frozen and WB
  • Reacts with bovine, dog, human, mouse, rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, IP, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Dog, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone MX823
Immunogen Synthetic c-terminal peptide (aa632-649) coupled to KLH
Formulation Contains 0.09% sodium azide
UniprotID Q16643 8 (Human)
Synonym Developmentally-regulated brain protein, DBN1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:50
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:100
Background

MX823 specifically reacts with drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000). It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and  carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
Reactivity on cultured cell lines: primary human keratinocytes, U333, MCF-7, SV80, B16 (mouse melanoma cells).

References/Publications (2)
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-Drebrin guinea pig polyclonal, serum
Cat. No: GP823
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC, IP and WB
  • Reacts with bovine, human, mouse and rat

$333.00*
anti-Drebrin guinea pig polyclonal, serum
Cat. No: GP254
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC, IP and WB
  • Reacts with bovine, human, mouse and rat

$333.00*