anti-Vasoactive Intestinal Peptide rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with cat, human, pig and rat
Product description
Quantity | 50 µl (lyoph.) |
---|---|
Antibody Type | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Application | IHC |
Purification | Undiluted antiserum |
Reactivity | Cat, Human, Pig, Rat |
Storage before reconstitution | 2-8°C until indicated expiry date |
Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Immunogen | Purified porcine VIP |
Formulation | Lyophilized; reconstitute in 100 µl dist. water |
UniprotID | A0A5F5XTM9 (Cat, Felis silvestris catus), P01282 (Human), P01284 (Pig), P01284 (Pig), P04566 (Guinea pig), P01283 (Rat) |
Synonym | VIP peptides [Cleaved into: Intestinal peptide PHV-42, Peptide histidine valine 42; Intestinal peptide PHM-27, Peptide histidine methioninamide 27; Vasoactive intestinal peptide, VIP, Vasoactive intestinal polypeptide], VIP |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | 1:1,500-1:5,000 |
Immunohistochemistry (IHC) - paraffin | 1:1,500-1:5,000 (microwave treatment recommended) |
Background
VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while PHI, secretin, glucagon, GIP, and CCK do not.
Positive control: formalin-fixed paraffin sections of cat ileum.
References/Publications (4)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.