anti-Vesicular Acetylcholine Transporter rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with mouse and rat
|Quantity||50 µl (lyoph.)|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|UniprotID||O35304 (Mouse),Q62666 (Rat)|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:800-1:1,200|
|Immunohistochemistry (IHC) - paraffin||1:800-1:1,200 (microwave treatment recommended)|
The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholine esterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining.
Positive control: frozen sections of rat small intestine.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Rabbit polyclonal
- Suitable for IHC on frozen sections
- Reacts with dog, guinea pig, hamster, human, mouse and rat