Epitope mapped
Frequently used
Diagnostic marker

anti-p53 Protein mouse monoclonal, Bp53.11, liquid, purified

Liquid

Lyophilized

Liquid

Cat. No: 690039

Available, delivery time 1-7 days

$425.00*

Key Features
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC,IP and WB
  • Reacts with human
  • Isotype: IgG2a
Product description
Quantity 1 ml (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ELISA, ICC/IF, IHC, IP, WB
Purification Affinity chromatography
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Bp53.11
Immunogen Recombinant human p53 (transcription domain within the NH2 terminus)
Concentration 50 µg/ml
Formulation PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
UniprotID Q96A56 (Human)
Synonym Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1, TP53INP1, P53DINP1, SIP
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:1,000-1:3,000
Immunohistochemistry (IHC) - paraffin 1:1,000-1:3,000 (microwave treatment recommended)
ELISA Assay dependent
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) Assay dependent
Background

Excellent marker for wild-type and mutant forms of human p53 antigen. Epitope recognized by Bp53.11: 20-SDLWKLLPENNV-31. The antibody stains positively approx. 60% of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors; T-cell Leukemia, non-Hodgkin-Lymphoma, melanoma, sarcoma.
Reactivity on cultured cell lines: Hela, MCF-7.

References/Publications (7)
Publication
Species
Application
Downloads
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Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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