- Consists of fully assembled, empty AAV6 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
Product description
| Quantity | 1 vial (1.0E+09-1.6E+09 capsids) |
|---|---|
| Application | ELISA |
| Storage | 2-8°C |
| Intended use | Research use only |
| Concentration | 2.0E+09-3.3E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial) |
| Formulation | Lyophilized, empty AAV6 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV6 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer. |
| Stability | 4 weeks at 2-8°C after reconstitution in ASSB 1x |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with PROGEN's AAV6 Titration ELISA) and analysis at least in duplicates is recommended. |
Background
The AAV6 ELISA Control consists of fully assembled, empty AAV6 capsids. The concentration is lot specific.
The AAV6 ELISA Control can be used as a positive control with PROGEN's AAV6 Titration ELISA (PRAAV6). If you require different lots of the AAV6 ELISA Control and the Kit Control included in your PROGEN AAV6 Titration ELISA, please enquire to check availability.
Reading of the AAV6 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV6 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
The AAV6 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAV6 capsids that has been characterized by qPCR and ddPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). For further information, please see as an example our poster The New AAV3 Titration ELISA.
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FAQs
Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.
ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.
Yes, PROGEN offers serotype-specific AAV ELISA Controls for the standardization of your AAV titer determination.
The serotype-specific positive controls are fully characterized empty AAV capsids standardized with PROGEN´s internal gold standards* (AAV1, AAV3, AAV5, AAV6, AAV9, AAVrh10 and AAVrh74) or the ATCC international gold standard material (AAV2 & AAV8).
*For more information on PROGEN´s internal gold standard, take a look at our posters "Developing Reliable AAV Standards for ELISA" and "The New AAV3 Titration ELISA" describing the establishment and characterization of AAV5 and AAV3 internal gold standards.
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