AAVrh10 empty capsids
SDS PAGE with AAVrh10 empty capsids. The AAVrh10 VP1, VP2 and VP3 proteins were separated on a 10% SDS PAGE and visualized by Pierce Silver stain kit (Cat. No. 24612). Only VP1, VP2 and VP3 proteins in the correct stoichiometry of 1:1:10 are detectable indicating a purity of the AAV preparation of >95%.
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SDS PAGE with AAVrh10 empty capsids. The AAVrh10 VP1, VP2 and VP3 proteins were separated on a 10% SDS PAGE and visualized by Pierce Silver stain kit (Cat. No. 24612). Only VP1, VP2 and VP3 proteins in the correct stoichiometry of 1:1:10 are detectable indicating a purity of the AAV preparation of >95%.
- Fully assembled, empty AAVrh10 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
Product description
| Quantity | 100 µl (> 5.0E+11 capsids) |
|---|---|
| Application | Dot blot, ELISA, WB |
| Purification | Affinity Chromatography (POROS CaptureSelect AAVX Affinity Resin, Thermo Fisher Scientific) |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Intended use | Research use only |
| Concentration | > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial |
| Formulation | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Source | Produced in HEK293T cells |
| Purity | > 95% by SDS-PAGE and silver stain |
| Quality check | Final titer was assigned on internal reference material using AAVrh10 ELISA kits (Cat. No. PRAAV10); QC included analysis of filling grade and Endotoxin testing |
| Packaging Plasmid | pRep2-Cap-rh10 + pHelper |
| Product description | Purified empty adeno-associated virus rh10 capsids (AAVrh10) |
| Endotoxin | < 1.0 EU/ml |
| Note | Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Dot Blot | Depending on primary antibody and detection method |
| ELISA | As a positive control in ELISA, dilute in ASSB 1x (provided with PROGEN’s ELISAs) and analysis at least in duplicates is recommended |
| Western Blot (WB) | Depending on primary antibody and detection method |
Background
The AAVrh10 empty capsids are provided with titers above 5.0E+12 capsids/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA.
Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.
PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 5, 6, 8, 9 and rh74.
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FAQs
The empty capsids are prepared in the absence of an ITR plasmid containing the AAV vector genome, so that no viral vector DNA can be packaged in the particles. In some rare cases the preparations may contain a very small amount of capsids in which fragments of the host cell genome and the packaging plasmids or other DNA fragments have been packaged.
Empty capsids (AAV1, 2, 5, 6, 8, 9, rh10 and rh74) are provided in liquid format in PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl without protein additives. The titer is greater than 5.0E+12 capsids/ml. Therefore, they can be used in several applications.
ELISA Kit Controls (AAV1, 2, 3, 5, 6, 8, 9, rh10 and rh74) are lyophilized. After reconstitution in ASSB 1x the final solution contains stabilizing protein, phenol red and ASSB 1x buffer. The titer is lot-specific around 7.9E+09-1.3E+10 capsids/ml. This product is mainly suitable for the ELISA.
The total capsid titer of our empty capsids is determined by PROGEN ELISA.
Analysis of the filling grade is performed by AUC or an orthogonal method such as UV/Vis.
Related Products
- ELISA for quantitation of AAVrh10 capsids
- Detection of full and empty AAVrh10 capsids
- ADK8 antibody is used as capture and detection antibody
- Consists of fully assembled, empty AAVrh74 capsids
- Represents serotype-specific conformational epitopes
- Reliable positive control for ELISA
- Fully assembled, empty AAVrh74 capsids
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA
- Quality controlled for purity, AAV titer and filling grade
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Fully assembled AAVrh74 capsids filled with an eGFP reporter gene controlled by a CMV promoter
- Serotype-specific conformational epitopes
- Reliable positive control for dot blot, western blot, ELISA, PCR and cell-based assays
- Quality controlled for purity, filling grade, aggregation, total capsid and genome titer
- Alignment to internal reference standard material
For US shipment, the packages are only sent out for delivery on Mondays and Tuesdays, in the EU from Monday until Wednesday.
- Purified, lyophilized
- Mouse monoclonal
- Suitable for dot blot, ELISA and neutralization
- Reacts with AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80 intact particles
- Isotype: IgG2a kappa
- Recombinant chimeric (human Fc region)
- Suitable for dot blot, neutralization assay and serology ELISA
- Reacts with AAV3, AAV7, AAV8, AAVrh10 and AAVrh74 intact capsids
- Isotype: IgG1
- Purified, liquid
- Mouse monoclonal
- Suitable for dot blot and ICC/IF
- Reacts with AAV8, AAVrh10, Anc80 and AAVrh74 intact particles
- Weak affinity for AAV9 and AAVDJ intact particles
- Isotype: IgG2a kappa
- Highly effective density gradient media for the separation of full and empty AAV particles
- Designed for the purification of viruses (such as AAV)
- Useful to isolate different cell types and organelles
- Ready-made, sterile and endotoxin-tested solution of iodixanol