Epitope mapped
Diagnostic marker
anti-Vimentin mouse monoclonal, XL-VIM-14.13, supernatant
Western blot analysis of HeLa lysate with anti-Vimentin antibody.
Western blot analysis was performed on 20 µg wild type (WT) and 20 µg Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal, XL-VIM-14.13 (Cat. No. 65189) was diluted in blocking buffer (1:10) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate. View more
Western blot analysis was performed on 20 µg wild type (WT) and 20 µg Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal, XL-VIM-14.13 (Cat. No. 65189) was diluted in blocking buffer (1:10) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate. View more
Western blot analysis of HeLa lysate with anti-Vimentin antibody.
Western blot analysis was performed on 20 µg wild type (WT) and 20 µg Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal, XL-VIM-14.13 (Cat. No. 65189) was diluted in blocking buffer (1:10) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
View moreWestern blot analysis was performed on 20 µg wild type (WT) and 20 µg Vimentin knockout (KO) HeLa lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Vimentin mouse monoclonal, XL-VIM-14.13 (Cat. No. 65189) was diluted in blocking buffer (1:10) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Key Features
- Mouse monoclonal
- Suitable for IEM, IHC and WB
- Reacts with huamn, carp and trout
- Isotype: IgG1
Product description
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IEM, IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Carp, Human, Trout |
| No reactivity | Bovine |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | XL-VIM-14.13 |
| Immunogen | Vimentin from cytoskeletal fraction of XLKE cells (cul-tured Xenopus laevis kidney epithelial cells) |
| Formulation | Contains 0.09% sodium azide |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | Ready-to-use |
| Cell-based Assay | Assay dependent |
| Western Blot (WB) | 1:10-1:20 |
Background
The anti-Vimentin antibody detects an epitope within the rod domain of xenopus and trout vimentin. Vimentin of amphibia and fish, predominantly found in glial and white blood cells.
Polypeptide reacting: MW 53,325 (pI 4.95) intermediate filament protein (vimentin) of Xenopus laevis (epitope presumably located between amino acids 79 and 88 within rod domain).
References/Publications (3)
Publication
Species
Application
Species
xenopus
Application
WB,ICC-IF,IEM
Species
trout
Application
WB,IHC (frozen)
Species
xenopus
Application
ICC-IF
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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