AAV8 VP1, recombinant protein


Western blot analysis of recombinant AAV2 VP proteins (Cat. No. 72001), recombinant AAV5 VP proteins (Cat. No. 72005), recombinant AAV6 VP proteins (Cat. No. 72006), recombinant AAV8 VP proteins (Cat. No. 72008) and recombiant AAV9 VP proteins (Cat. No. 72009) with B1 antibody (Cat. No. 690058). Western blot analysis was performed on the precise molar ratio of 1:1:10 (13.5 ng VP1:11 ng VP2:100 ng VP3) combined in one lane. The PVDF membrane was blocked with 5% milk in PBST for 1 h at RT.
The primary antibody anti-AAV VP1/VP2/VP3, B1 (Cat. No. 690058) was diluted in blocking buffer (antibody concentration 500 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.



Undiluited = 100 µg/ml, pre-diluted = 10 µg/ml













Western blot analysis of recombinant AAV2 VP proteins (Cat. No. 72001), recombinant AAV5 VP proteins (Cat. No. 72005), recombinant AAV6 VP proteins (Cat. No. 72006), recombinant AAV8 VP proteins (Cat. No. 72008) and recombiant AAV9 VP proteins (Cat. No. 72009) with B1 antibody (Cat. No. 690058). Western blot analysis was performed on the precise molar ratio of 1:1:10 (13.5 ng VP1:11 ng VP2:100 ng VP3) combined in one lane. The PVDF membrane was blocked with 5% milk in PBST for 1 h at RT.
The primary antibody anti-AAV VP1/VP2/VP3, B1 (Cat. No. 690058) was diluted in blocking buffer (antibody concentration 500 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG HRP was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.



Undiluited = 100 µg/ml, pre-diluted = 10 µg/ml











• Useful in combination with AAV8 VP2 and VP3 for ratio analysis (also available as set, Cat. No. 72008)
• Precise molar concentration
• Suitable for WB, dot blot, SDS PAGE
Product description
Quantity | 10 µg |
---|---|
Application | Dot blot, SDS PAGE, WB |
Purification | Ni-NTA chromatography |
Storage | -80°C |
Intended use | Research use only |
Concentration | 100 µg/ml (1.19 µM) |
Formulation | Liquid, 6 M urea in PBS |
Source | Escherichia coli |
Molecular weight | 83.9 kDa (calculated Mw from aa sequence) |
Purity | > 95% (determined by SDS PAGE) |
Product description | N-terminal His-tagged (MGSSHHHHHHSSGLVPRGSH) recombinant AAV8 capsid protein VP1 |
Applications
Tested applications | Tested dilutions |
---|---|
Dot Blot | 100 ng, depending on primary antibody and detection method |
SDS PAGE | 1 µg |
Western Blot (WB) | 5-20 ng, depending on primary antibody and detection method |
Background
All three recombinant AAV8 capsid proteins are available as set (Cat. No. 72008) or as individual proteins (Cat. No. 640839, 640840, 640841).
Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
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