Disease related
Frequently used

anti-Plakophilin 1 mouse monoclonal, PP1-5C2, supernatant

Supernatant

Sample

Cat. No: 65160

Available, delivery time 1-7 days

$245.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine, human, mouse, pig and rat
  • Isotype IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human, Mouse, Pig, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone PP1-5C2
Immunogen Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
Formulation Contains 0.09% sodium azide
UniprotID Q28161 (Bovine), Q13835 (Human), P97350 (Mouse), I3LGN8 (Pig), D3ZY51 (Rat)
Synonym Plakophilin-1, Band 6 protein, B6P, PKP1
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Undiluted-1:50 (preincubation with 0.05-0.2% Triton X-100, for 5-10 min recommended; for delicate tissues like liver and gastrointestinal material preincubation with saponin is preferred)
Immunohistochemistry (IHC) - paraffin Undiluted-1:50 (pretreatment with 0.001% trypsin and microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) Assay dependent
Background

Antibody recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative.

Polypeptide reacting: Plakophilin 1: calculated mol. wt. 80,496 (human) and 80,180 (bovine).

Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue).

References/Publications (8)
Publication
Species
Application
Downloads
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Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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